Autocrine ligand binding to cell receptors. Mathematical analysis of competition by solution "decoys"
نویسندگان
چکیده
منابع مشابه
Analysis of ligand binding to receptors by competition kinetics. Application to muscarinic antagonists in rat brain cortex.
We propose a method for obtaining kinetic parameters of unlabeled ligands which is based on analyzing the effect of their competition on the binding kinetics of a labeled ligand. In this method (competition kinetics), the binding kinetics of a labeled ligand are measured in the absence and in the presence of given concentrations of a competing unlabeled ligand. The rate equations appropriate to...
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Nuclear receptors play an important role in transcription regulation. They bind as homoor heterodimers to the response elements of their target genes and interact with numerous and diverse partners, e. g. coactivator and corepressor proteins, and transcription factors. Many of these processes are ligand-dependent, i. e. binding of natural ligands activates the nuclear receptor through conformat...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1992
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(92)81856-8